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Title: Assignment<FOOTREF>[sup 1] </FOOTREF> of SH3KBP1 to human chromosome band Xp22.1→p21.3 by in situ hybridization.
Authors: Narita, T.; Amano, F.; Yoshizaki, K.; Nishimoto, N.; Nishimura, T.; Tajima, T.; Namiki, H.; Taniyama, T.
Abstract: Lymphocytes isolated from human peripheral blood were cultured in α minimal essential medium (α -MEM) supplemented with 10% fetal bovine serum and phytohemagglutinin (PHA) at 37°C for 68-72 h. The lymphocyte cultures were treated with BrdU to synchronize the cell population. The DNA probe was biotinylated with dATP using the Gibco BRL BioNick labeling kit and procedure for FISH detection was performed. The results showed that the number of cells with specific signal were 1(3), 2(70), chromatids per cell.
Subjects: GENETICS; GENES; HUMAN chromosomes; IN situ hybridization; LYMPHOCYTES; PHYTOHEMAGGLUTININS
Publication: Cytogenetics & Cell Genetics, 2001, Vol 93, Issue 1/2, p133
ISSN: 0301-0171
Publication type: Academic Journal
DOI: 10.1159/000056966

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Assignment<FOOTREF>[sup 1] </FOOTREF> of SH3KBP1 to human chromosome band Xp22.1→p21.3 by in situ hybridization.