Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology.

Jia Jia; Xiaofeng Yang; Zhiliang Wu; Qian Zhang; Zhi Lin; Hongtao Guo; Carol Sze Ki Lin; Jianying Wang; Yunshan Wang; Jia, Jia; Yang, Xiaofeng; Wu, Zhiliang; Zhang, Qian; Lin, Zhi; Guo, Hongtao; Lin, Carol Sze Ki; Wang, Jianying; Wang, Yunshan

Lipase produced by Aspergillus niger is widely used in various industries. In this study, extracellular lipase production from an industrial producing strain of A. niger was improved by medium optimization. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments. According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil. Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium. All these results confirmed the validity of the model. Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.

FERMENTATION; LIPASES; ASPERGILLUS niger; RESPONSE surfaces (Statistics); CARBON; NITROGEN; LIPIDS

BioMed Research International, 2015, Vol 2015, p1

2314-6133

Academic Journal

10.1155/2015/497462