Green Tea Epigallocatechin Gallate Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via the Inhibition of Both Focal Adhesion Kinase and Insulin-Like Growth Factor-I Receptor.

Hoang Anh Vu; Yuuichi Beppu; Hoang Thanh Chi; Kousuke Sasaki; Hideaki Yamamoto; Phan Thi Xinh; Takashi Tanii; Yukihiko Hara; Toshiki Watanabe; Yuko Sato; Iwao Ohdomari

The exact molecular mechanism by which epigallocatechin gallate (EGCG) suppresses human pancreatic cancer cell proliferation is unclear. We show here that EGCG-treated pancreatic cancer cells AsPC-1 and BxPC-3 decrease cell adhesion ability on micropattern dots, accompanied by dephosphorylations of both focal adhesion kinase (FAK) and insulin-like growth factor-1 receptor (IGF-1R) whereas retained the activations of mitogen-activated protein kinase and mammalian target of rapamycin. The growth of AsPC-1 and BxPC-3 cells can be significantly suppressed by EGCG treatment alone in a dose-dependent manner. At a dose of 100 μM which completely abolishes activations of FAK and IGF-1R, EGCG suppresses more than 50% of cell proliferation without evidence of apoptosis analyzed by PARP cleavage. Finally, the MEK1/2 inhibitor U0126 enhances growth-suppressive effect of EGCG. Our data suggests that blocking FAK and IGF-1R by EGCG could prove valuable for targeted therapy, which can be used in combination with other therapies, for pancreatic cancer.

EPIGALLOCATECHIN gallate; CANCER cell proliferation; PANCREATIC cancer; ANTINEOPLASTIC agents; GREEN tea; FOCAL adhesion kinase; SOMATOMEDIN C; INSULIN-like growth factor receptors; PREVENTION

Journal of Biomedicine & Biotechnology, 2010, p1

1110-7243

Academic Journal

10.1155/2010/290516