Patterned substrates, coupled with surfactant addition, offer a way to exercise more control in the convective self-assembly of colloidal nanospheres. In this work, we investigated the surfactant effects on dynamic colloidal self-assembly during evaporation in a sessile drop. The real time video monitoring revealed that the surfactant plays a dominating role to determine the wetting angle of the colloidal suspension in a sessile drop. Meanwhile, surfactant also had great influence on the cluster formation during assembly process and the final lattice structure.