Coriandrum sativum Suppresses Aβ42-Induced ROS Increases, Glial Cell Proliferation, and ERK Activation.

Liu, Quan Feng; Jeong, Haemin; Lee, Jang Ho; Hong, Yoon Ki; Oh, Youngje; Kim, Young-Mi; Suh, Yoon Seok; Bang, Semin; Yun, Hye Sup; Lee, Kyungho; Cho, Sung Man; Lee, Sung Bae; Jeon, Songhee; Chin, Young-Won; Koo, Byung-Soo; Cho, Kyoung Sang

Alzheimer's disease (AD), the most common neurodegenerative disease, has a complex and widespread pathology that is characterized by the accumulation of amyloid -peptide (A) in the brain and various cellular abnormalities, including increased oxidative damage, an amplified inflammatory response, and altered mitogen-activated protein kinase signaling. Based on the complex etiology of AD, traditional medicinal plants with multiple effective components are alternative treatments for patients with AD. In the present study, we investigated the neuroprotective effects of an ethanol extract of Coriandrum sativum ( C. sativum) leaves on A cytotoxicity and examined the molecular mechanisms underlying the beneficial effects. Although recent studies have shown the benefits of the inhalation of C. sativum oil in an animal model of AD, the detailed molecular mechanisms by which C. sativum exerts its neuroprotective effects are unclear. Here, we found that treatment with C. sativum extract increased the survival of both A-treated mammalian cells and 42-expressing flies. Moreover, C. sativum extract intake suppressed -induced cell death in the larval imaginal disc and brain without affecting A42 expression and accumulation. Interestingly, the increases in reactive oxygen species levels and glial cell number in AD model flies were reduced by C. sativum extract intake. Additionally, C. sativum extract inhibited the epidermal growth factor receptor- and A-induced phosphorylation of extracellular signal-regulated kinase (ERK). The constitutively active form of ERK abolished the protective function of C. sativum extract against the -induced eye defect phenotype in Drosophila. Taken together, these results suggest that C. sativum leaves have antioxidant, anti-inflammatory, and ERK signaling inhibitory properties that are beneficial for patients with AD.

SOUTH Korea; AMYLOIDOSIS; REACTIVE oxygen species; ALTERNATIVE medicine; ANIMAL experimentation; ANTI-inflammatory agents; ANTIOXIDANTS; BIOLOGICAL assay; BIOLOGICAL models; BRAIN; CELL death; CELL receptors; EPIDERMAL growth factor; EYE; IMMUNOHISTOCHEMISTRY; INSECTS; INSECT larvae; LEAVES; MEDICINAL plants; NEURONS; PEPTIDES; PHOSPHORYLATION; POLYMERASE chain reaction; PROTEIN kinases; RESEARCH funding; STAINS & staining (Microscopy); WESTERN immunoblotting; PLANT extracts; REVERSE transcriptase polymerase chain reaction; IN vitro studies; IN vivo studies; PREVENTION

American Journal of Chinese Medicine, 2016, Vol 44, Issue 7, p1325

0192-415X

Academic Journal

10.1142/S0192415X16500749