Method for Primary Screening of Pharmaceuticals on the Paramecium caudatum Eukaryotic Cell Model.

Gruzdev, G. A.; Karpukhina, O. V.; Inozemtsev, A. N.; Kamensky, A. A.

The effect of adrenaline in various concentrations and dopamine at a concentration of 10–10 mol/mL on the behavior of Paramecium caudatum was studied. It is shown that adrenaline reduces motor activity and changes the movement strategy of these protozoans; a dose-dependent behavioral response on the drug concentration was observed. This effect can be explained by the presence of adrenaline receptors located on the surface of the cell membrane. To study the direct effect of adrenaline on alpha and beta adrenergic receptors, the effect of non-selective adrenoblockers nicergoline and timolol is considered in this paper. At the same time, dopamine at a concentration of 10–10 mol/mL does not have a significant effect on the nature and magnitude of motor activity during the entire registration time, since this organism does not have receptors for this mediator. The proposed method makes it possible to quickly and objectively assess the nature of the effects of various pharmaceuticals acting on the catecholamine system.

BETA adrenoceptors; ADRENERGIC receptors; EUKARYOTIC cells; CELL receptors; PARAMECIUM; DOPAMINE receptors

Doklady Biochemistry & Biophysics, 2022, Vol 502, Issue 1, p36

1607-6729

Academic Journal

10.1134/S1607672922010033