Crystal structure of a mutant of archaeal ribosomal protein L1 from Methanococcus jannaschii.

Sarskikh, A.; Gabdulkhakov, A.; Kostareva, O.; Shklyaeva, A.; Tishchenko, S.

The crystal structure of a mutant of archaeal ribosomal protein L1 from Methanococcus jannaschii with the deletion of a nonconserved positively charged cluster consisting of eight C-terminal amino acid residues is determined by the molecular replacement method at 1.75 Å resolution. This mutant is shown to form more stable and ordered crystals belonging to a space group other than that of the wild-type protein crystals. The positively charged C-terminal region has only a slight effect on the interaction between protein L1 and RNA molecules. Hence, this mutant can be used to prepare protein-RNA complexes and obtain their crystals.

BACTERIAL protein crystallography; ARCHAEBACTERIA; RIBOSOMAL proteins; BACTERIAL protein structure; METHANOCALDOCOCCUS jannaschii; C-terminal residues; AMINO acid residues

Crystallography Reports, 2014, Vol 59, Issue 3, p394

1063-7745

Academic Journal

10.1134/S1063774514030158