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Title

Critical chromatography of macromolecules as a tool for reading the amino acid sequence of biomacromolecules: Reality or science fiction?

Authors

Gorshkov, A. V.; Evreinov, V. V.; Tarasova, I. A.; Gorshkov, M. V.

Abstract

The capabilities of critical chromatography to study the amino acid sequences in biopolymer, peptide, and protein macromolecules are discussed. The rearrangement of two or more amino acid residues and the occurrence and location of modified residues in a chain are considered. The mechanism of an inversion in the order of peptide elution under changes in the gradient of the solvent composition is discussed.

Subjects

AMINO acid sequence; BIOPOLYMERS; CHROMATOGRAPHIC analysis; PEPTIDES; PROTEIN analysis

Publication

Journal of Analytical Chemistry, 2010, Vol 65, Issue 1, p2

ISSN

1061-9348

Publication type

Academic Journal

DOI

10.1134/S1061934810010028

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