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- Title
The Genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma.
- Authors
Sinkov, V. V.; Orlova, E. A.; Ogarkov, O. B.; Suzdalnitsky, A. E.; Kondratov, I. G.; Belkova, N. L.; Rychkova, L. V.; Kolesnikova, L. I.
- Abstract
A number of facultative-anaerobic lipophilic microorganisms, including representatives of Corynebacterium and Staphylococcaceae, inhabit the necrotic contents of tuberculomas. A strain of Staphylococcus epidermidis was isolated from caseum; its genome was fully sequenced and gene-mapped. The coagulase-negative Staphylococcus belonged to the MLST 73 genotype and was resistant to two antituberculosis drugs. The strain phenotypically had urease, gelatinase, and beta-hemolytic activities and possessed the corresponding genes. Similar to S. epidermidis O47, its genome consists of a single chromosome containing approximately 2.4 million base pairs, and oriC has the same orientation. A total of 2333 genes were identified, of which 2206 were coding genes. In contigs of the genome, sequences of plasmid replication genes were found: rep7a, rep13, rep5b, and pSK1. Phylogenetic analysis indicates the closeness of the analyzed genome to a large group of European strains. Considering the biochemical and microbiological properties of the isolated strain, we hypothesize that staphylococci and other facultative-anaerobic satellite microorganisms of tuberculosis foci may play an important role in caseum liquefaction owing to their own proteolytic activity and attraction of neutrophils to the focus of inflammation.
- Subjects
STAPHYLOCOCCUS epidermidis; ANTITUBERCULAR agents; GENE mapping; TUBERCULOMA; BASE pairs; MYCOBACTERIUM tuberculosis
- Publication
Russian Journal of Genetics, 2024, Vol 60, Issue 10, p1451
- ISSN
1022-7954
- Publication type
Academic Journal
- DOI
10.1134/S1022795424701011