The Genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma.

Sinkov, V. V.; Orlova, E. A.; Ogarkov, O. B.; Suzdalnitsky, A. E.; Kondratov, I. G.; Belkova, N. L.; Rychkova, L. V.; Kolesnikova, L. I.

A number of facultative-anaerobic lipophilic microorganisms, including representatives of Corynebacterium and Staphylococcaceae, inhabit the necrotic contents of tuberculomas. A strain of Staphylococcus epidermidis was isolated from caseum; its genome was fully sequenced and gene-mapped. The coagulase-negative Staphylococcus belonged to the MLST 73 genotype and was resistant to two antituberculosis drugs. The strain phenotypically had urease, gelatinase, and beta-hemolytic activities and possessed the corresponding genes. Similar to S. epidermidis O47, its genome consists of a single chromosome containing approximately 2.4 million base pairs, and oriC has the same orientation. A total of 2333 genes were identified, of which 2206 were coding genes. In contigs of the genome, sequences of plasmid replication genes were found: rep7a, rep13, rep5b, and pSK1. Phylogenetic analysis indicates the closeness of the analyzed genome to a large group of European strains. Considering the biochemical and microbiological properties of the isolated strain, we hypothesize that staphylococci and other facultative-anaerobic satellite microorganisms of tuberculosis foci may play an important role in caseum liquefaction owing to their own proteolytic activity and attraction of neutrophils to the focus of inflammation.

STAPHYLOCOCCUS epidermidis; ANTITUBERCULAR agents; GENE mapping; TUBERCULOMA; BASE pairs; MYCOBACTERIUM tuberculosis

Russian Journal of Genetics, 2024, Vol 60, Issue 10, p1451

1022-7954

Academic Journal

10.1134/S1022795424701011