Results
Title

Molecular Cloning, Characterization and Expression Pattern of Two RARs and a RAR-RXRγ Genes in Carassius auratus.

Authors

Li, Q.; Li, L.; He, B.

Abstract

As members of nuclear hormone receptor superfamily, retinoic acid receptor and retinoid X receptors are involved in the transduction of retinoic acid signaling. In this study, the full cDNA length of Ca-RARα, Ca-RARγ and Ca-RAR-RXRγ genes were cloned in Carassius auratus. Both Ca-RARα and Ca-RARγ proteins contain the AF-1, AF-2, P-box, D-box, DBD and LBD conservative domains, with 63.09% similarity, but the location of AF-2 in two isoforms is different, with located the end of LBD region in Ca-RARα, and behind the LBD region in Ca-RARγ. Phylogenetic tree indicated that RAR-RXRγ had more closely related to RARα than RARγ. Then the three-dimensional structure of Ca-RARα, Ca-RARγ and Ca-RAR-RXRγ proteins were constructed according to the highest identity with the Homo sapiens RARβ protein. Ca-RARα, Ca-RARγ and Ca-RAR-RXRγ mRNA in several tissues of C. auratus were determined byquantitative real-time PCR analysis. The results shown that Ca-RARα and Ca-RARγ mRNA present tissue-specific expression pattern, with the highest level of Ca-RARα mRNA in ovary, and that of Ca-RARγ mRNA in head kidney. However, Ca-RAR-RXRγ mRNA expression had a widespread distribution, with the highest level in the head kidney, then followed by heart, ovary, intestines, skin, blood and gill. This study lays a foundation for further explore the molecular regular mechanism of vitamin A by RA signaling.

Subjects

GENE expression; GOLDFISH; MOLECULAR cloning; RETINOIC acid receptors; RETINOID X receptors; NUCLEAR receptors (Biochemistry); VITAMIN A

Publication

Russian Journal of Genetics, 2023, Vol 59, p61

ISSN

1022-7954

Publication type

Academic Journal

DOI

10.1134/S1022795423130094