Yermishin, A. P.; Levy, A. V.; Ageeva, A. S.; Voronkova, E. V.; Luksha, V. I.; Gukasian, O. N.; Zharich, V. M.

doi:10.1134/S1022795423070050

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Title: Specifics of Transfer of DNA Markers of Wild Allotetraploid Potato Species Solanum stoloniferum to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression.
Authors: Yermishin, A. P.; Levy, A. V.; Ageeva, A. S.; Voronkova, E. V.; Luksha, V. I.; Gukasian, O. N.; Zharich, V. M.
Abstract: Genomic difference between wild allotetraploid potato species Solanum stoloniferum Schltdl et Bouchet (genome AABB) and S. tuberosum L. (AAAA) is one of the factors hampering its use in breeding. However, there is practically no information on valuable genes of this species located in the subgenome B and on the way of their transfer into the genome A of cultivated potatoes. The objectives of this research were to identify subgenomic location of a set of S. stoloniferum genes using an original approach based on a difference of inheritance of DNA markers of the genes in backcross BC1 of chromosome doubled triploid hybrids (6x, AAAABB) to 4х S. tuberosum dependent on belonging to A or B subgenome; to study their inheritance in BC2 and BC3 in the framework of four introgression schemes and marker assisted selection of the gene Rpi-sto1. The markers of late blight (LB) resistance genes Rpi-sto1, R3b, R2, potato virus Y (PVY) resistance genes Rysto,Ryadg were located on subgenome B and the marker of PVY resistance gene Rychc—on subgenome A. We observed an appearance of unexpected sporadic hybrids free of markers in BC1 that may be explained by rare cases of homeological recombination of A and B subgenome chromosomes. The segregation of the markers in BC2 (close to 1 : 1) matched in general to that expected in the case of random transfer of the corresponding chromosomes of the subgenome B. Some promising for breeding hybrids have been selected in BC3 having the marker of the gene Rpi-sto1.
Subjects: INTROGRESSION (Genetics); GENETIC markers; PLANT hybridization; POTATO virus Y; CHROMOSOMES; SOLANUM; HEREDITY; POTATOES; SPECIES
Publication: Russian Journal of Genetics, 2023, Vol 59, Issue 7, p642
ISSN: 1022-7954
Publication type: Academic Journal
DOI: 10.1134/S1022795423070050

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Specifics of Transfer of DNA Markers of Wild Allotetraploid Potato Species Solanum stoloniferum to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression.

Yermishin, A. P.; Levy, A. V.; Ageeva, A. S.; Voronkova, E. V.; Luksha, V. I.; Gukasian, O. N.; Zharich, V. M.

INTROGRESSION (Genetics); GENETIC markers; PLANT hybridization; POTATO virus Y; CHROMOSOMES; SOLANUM; HEREDITY; POTATOES; SPECIES

Russian Journal of Genetics, 2023, Vol 59, Issue 7, p642

1022-7954

Academic Journal

10.1134/S1022795423070050