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- Title
Cytogenetic and Karyotype Study on Hibiscus sabdariffa L. in Indigenous Populations of Iran.
- Authors
Salehi, B.; Pazuki, A.; Zargari, K.
- Abstract
The main goal of cytogenetic studies on a plant is to provide general information about the evolutionary history of the plant, determine its cytological characteristics, and find resemblances between the case study and other plants such as species affinities. Some of this information is necessary for achieving plant breeding goals and determining appropriate policies. So far there have been no cytogenetic and karyological studies in different populations of the medicinal plant of sour tea in Iran. Thus, in this study, three different populations of Roselle (Hibiscus sabdariffa L.), obtained from Isfahan, Shiraz, and Sistan and Baluchestan, were studied on the basis of seven chromosomal parameters. The chromosomal data were analyzed in a completely randomized design with 10 replications and 5 observations. The three different populations were compared in terms of cytogenetical charactristics. According to the results of the analysis of variance table, there is no significant or meaningful difference in terms of these seven parameters among the three different populations of roselle. However, karyotypic diversity can be the source of genetic variation. Thus, the selection of parental bases for intraspecific and interspecific crossings should be based on the greater similarity of karyotypes in terms of the mentioned parameters. In all three populations the longest chromosome is chromosome no. 1 and the shortest one is chromosome no. 18. In the Isfahan, Shiraz, and Sistan and Baluchestan populations, chromosome no. 1 has the average length of 4.75, 4.74, and 4.69 µm, respectively. Chromosome no. 18 has the average length of 1.64 in both the Isfahan and Shiraz populations, and the average length of 1.65 in the Sistan and Baluchestan population. Moreover, the average chromatin length in the Isfahan population is equal to 46.39 µm, in the Shiraz population equal to 46.41 µm, and in the Sistan and Baluchestan population equal to 46.27 µm. The general formula was the same among the different populations and they were placed in the same chromosomal class. All three populations have the karyotype formula 18m with no observed satellites. The results showed that all of the studied populations were tetraploid (2n = 4x = 72). According to Stebbins (1971) method of classification, all populations were classified as 3B symmetry. Also using flow cytometry method, the average amount of nuclear DNA content in Isfahan and Shiraz populations, 5.7 picograms were calculated.
- Subjects
ROSELLE; INDIGENOUS peoples; KARYOTYPES; NUCLEAR DNA; PLANT breeding; PLANT populations
- Publication
Russian Journal of Genetics, 2022, Vol 58, Issue 4, p413
- ISSN
1022-7954
- Publication type
Academic Journal
- DOI
10.1134/S1022795422040123