Role of lipoxygenase and allene oxide synthase in wound-inducible defense response of pea.

Yang, H.; Liu, H.; Tang, K.; Huang, W.

Lipoxygenase (LOX) and allene oxide synthase (AOS) are key enzymes in the jasmonic acid (JA) biosynthesis pathway. In this work, the role of LOX and AOS in defense response induced by wounding was investigated using pea ( Pisum sativum L., cv. Ning Xia) seedlings as the material. The results showed that wound-induced 'JA burst' was accompanied by the activation of LOX and AOS and the accumulation of their mRNAs; applied JA also stimulated the accumulation of LOX and AOS. Further experiments conducted with inhibitors demonstrated that the wound-induced JA was regulated by LOX and AOS at both transcriptional and enzymic levels. Their activation was necessary in wound-mediated defense response and could enhance the tolerance of pea seedlings to wounding.

PLANT defenses; PLANT injuries; PEAS; LIPOXYGENASES; ALLENE; JASMONIC acid; BIOACCUMULATION; ENZYME kinetics

Russian Journal of Plant Physiology, 2011, Vol 58, Issue 2, p238

1021-4437

Academic Journal

10.1134/S1021443711020233