Title: On the Application of the Critical Chromatography Concept for Investigation of the Nucleotide Sequence of Single-Stranded DNA.
Authors: Gorshkov, A. V.; Rozdina, I. G.; Evreinov, V. V.
Abstract: Lattice model of macromolecules adsorption has been applied to describe the separation of single-stranded DNA on a reversed phase in a water–acetonitrile mixture under isocratic and gradient modes. It has been shown that this model, extended with correlation relationships between the effective unit energy and the solvent composition, can accurately describe the features of chromatographic separation of homo- as well as heteropolynucleotides. The correlation between the calculated and experimental data has been less accurate in the case of the heteropolymers in comparison with homopolymers, due to low contribution of the entropy term of the adsorption interaction with variation of the sequence. The approaches to the model adjustment to account for the neighbor units effect have been discussed.
Subjects: NUCLEOTIDE sequence; DNA sequencing; SINGLE-stranded DNA; CHROMATOGRAPHIC analysis; HETEROCHAIN polymers; MACROMOLECULES
Publication: Polymer Science -- Series A, 2022, Vol 64, Issue 1, p63
ISSN: 0965-545X
Publication type: Academic Journal
DOI: 10.1134/S0965545X22010035

On the Application of the Critical Chromatography Concept for Investigation of the Nucleotide Sequence of Single-Stranded DNA.

Gorshkov, A. V.; Rozdina, I. G.; Evreinov, V. V.