Applicability of the critical-chromatography concept to proteomics problems: Separation of peptides modeled by a heterogeneous rod.

Gorshkov, A.; Goloborodko, A.; Pridatchenko, M.; Tarasova, I.; Rozdina, I.; Evreinov, V.; Gorshkov, M.

The problems of separation of short peptides are considered in terms of the model of rigid rod adsorption. Analytical expressions relating the retention volume to the sequence of amino-acid residues in the gradient elution are obtained. The model of adsorption of a peptide as a rigid rod is compared with the model of its adsorption as a random-walk chain. The transition of rodlike peptides to the adsorbed state is more abrupt compared with the random-walk chain having the same sequence, while with an increase in the peptide length the random-walk chain model becomes more accurate. It is shown that the model of adsorption of a peptide as a rigid rod for short peptides fits the experimental data and correctly predicts the character of separation of peptides having equal lengths and identical amino-acid compositions but slightly differing in the alternation of residues in a chain.

INHOMOGENEOUS materials; CHROMATOGRAPHIC analysis; PEPTIDES; ADSORPTION (Chemistry); AMINO acids

Polymer Science -- Series A, 2017, Vol 59, Issue 3, p451

0965-545X

Academic Journal

10.1134/S0965545X17030063