Research of the Expression of IL-13 Receptors and IL-13R Genes in the Bronch of Rats with Ovalbumin-Induced Bronchial Asthma.

Blazhevich, L. E.; Smirnova, O. E.; Kirilina, V. M.; Masliukov, P. M.

This article presents the results of studies of the levels of expression of the IL13R gene and the levels of expression of the molecular IL13R in the bronchi of control rats and rats with ovalbumin-induced asthma. The choice of these receptors is due to the great importance of these structures in the pathogenesis of allergic bronchial asthma. Biological materials of 64 Wistar rats were studied using real-time PCR and immunohistochemical methods. As a result of the studies, it was found that under conditions of asthma development in the tissues of the bronchi of rats, a pronounced expression of the IL13R genes, a significant expression of the molecular receptor for interleukin-13, occurs. In bronchi with an intramural ganglion (bifurcation zone), the level of IL13R gene expression and the level of expression of the IL13R molecular receptor were significantly higher than in tracheal samples without ganglia. Sodium cromoglycate, administered to rats 5 hours after the last inhalation of ovalbumin, led to a decrease in the mRNA content in the bronchi of animals compared to animals that did not receive mast cell stabilizer treatment.

GENE expression; ASTHMA; CROMOLYN sodium; LABORATORY rats; RATS

Journal of Evolutionary Biochemistry & Physiology, 2023, Vol 59, Issue 2, p630

0022-0930

Academic Journal

10.1134/S002209302302028X