Two-dimensional electrophoretic proteome study of serum thermostable fraction from patients with various tumor conditions.

Goufman, E. I.; Moshkovskii, S. A.; Tikhonova, O. V.; Lokhov, P. G.; Zgoda, V. G.; Serebryakova, M. V.; Toropygin, I. Yu.; Vlasova, M. A.; Safarova, M. R.; Makarov, O. V.; Archakov, A. I.

One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, α-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin α1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis.

ELECTROPHORESIS; ELECTROCHEMISTRY; TUMORS; PROTEOMICS; MOLECULAR biology

Biochemistry (00062979), 2006, Vol 71, Issue 4, p354

0006-2979

Academic Journal

10.1134/S000629790604002X