Single and multiple sevoflurane exposures during pregnancy and offspring behavior in mice.

Lee, Soomin; Chung, Woosuk; Park, Haram; Park, Hanwool; Yoon, Seunghwan; Park, Sangil; Park, Jiho; Heo, Jun Young; Ju, Xianshu; Yoon, Seok ‐ hwa; Kim, Yoon Hee; Ko, Youngkwon; Vutskits, Laszlo

Background The second trimester is a period of neurogenesis and neuronal migration, which can be affected by exposure to anesthetics. Studies also suggest that multiple exposures may have a greater impact on neurodevelopment. Aim We investigated whether in utero single or multiple exposures to anesthetics caused long-term behavior changes. Methods Pregnant mice were randomly divided into four groups on gestational day 14 ( GD 14). Mice in the Control × 1 group were exposed to 100% oxygen for 150 min. Mice in the Sevo × 1 group were also exposed to 100% oxygen for 150 min, except that 2.5% sevoflurane was added during the first 120 min. Mice in the Control × 3 and Sevo × 3 group were identically treated as Control × 1 and Sevo × 1 group for three consecutive days, respectively ( GD 14-16). Behavioral tests were performed only with the male offspring at the age of 2-4 months. Synaptic plasticity was also compared by inducing long-term potentiation in acute hippocampal slices. Results Single or multiple sevoflurane exposures in pregnant mice during the second trimester did not cause long-lasting behavioral consequences or changes in long-term synaptic plasticity of their offspring. Conclusion Our study suggests that neither single nor multiple exposures of mice to sevoflurane during the fetal developmental period induces long-term behavioral dysfunctions or affects long-term synaptic plasticity. Additional studies focusing on early stages of neurodevelopment are necessary to confirm the effects of sevoflurane exposure during pregnancy.

SEVOFLURANE; PREGNANCY; DEVELOPMENTAL neurobiology; BEHAVIOR disorders in children; PEDIATRIC anesthesia; THERAPEUTICS

Pediatric Anesthesia, 2017, Vol 27, Issue 7, p742

1155-5645

Academic Journal

10.1111/pan.13139