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- Title
Acrofolione A and B, acetophenone dimers from Acronychia pendunculata, induce an apoptotic effect on human NALM‐6 pre‐B cell leukaemia cells.
- Authors
Matsui, Takuya; Ito, Chihiro; Kato, Ayumi; Wu, Tian‐Shung; Itoigawa, Masataka
- Abstract
Objectives: We investigated the apoptotic activities of acrofolione A (1) and B (2) isolated from Acronychia pedunculata against a human pre‐B cell leukaemia cell line (NALM‐6) to explore the apoptosis‐related signalling molecules targeted by 1 and 2. Methods: The apoptosis effects of 1 and 2 in NALM‐6 cells were investigated by TUNEL staining, annexin V, mitochondria membrane potential and caspase 3/7 activity. We carried out a protein array to explore the signalling molecules involved in apoptosis comprehensively. Key findings: Acrofolione A (1) suppressed the growth of NALM‐6, K562 and HPB‐ALL cells (IC50 16.7 ± 1.9, 17.9 ± 0.3 and 10.1 ± 0.2 μm, respectively) more effectively than acrofolione B (2). Both compounds time‐dependently increased the number of NALM‐6 cells with abnormal nuclei, and increased the number of annexin V‐positive cells and decreased the mitochondrial membrane potential of NALM‐6 cells. Acrofolione A (1) markedly elevated caspase 3/7 activity and increased the number of TUNEL‐positive cells. Cells treated with either compound showed enhanced expression of cleaved PARP and cleaved caspase 3 and 7, and reduced survivin protein levels. Conclusions: Acrofolione A (1) and B (2) may be useful in the treatment of various types of leukaemia.
- Subjects
ACETOPHENONE; RUTACEAE; APOPTOSIS; B cell lymphoma; MITOCHONDRIAL DNA
- Publication
Journal of Pharmacy & Pharmacology, 2019, Vol 71, Issue 3, p348
- ISSN
0022-3573
- Publication type
Academic Journal
- DOI
10.1111/jphp.13035