Discovery of two novel tilapia lake virus-like virus isolates in the transcriptomic data of guppy fish (Poecilia reticulata) Keywords: amnoonvirus; guppy fish; tilapia lake virus; TiLV; transcriptomic data reanalysis EN amnoonvirus guppy fish tilapia lake virus TiLV transcriptomic data reanalysis 1015 1019 5 08/11/23 20230901 NES 230901 INTRODUCTION Next-generation sequencing technology has revolutionized the era of modern microbiology by identifying novel viruses without prior knowledge of the nucleotide sequences present in the clinical samples (Bassi et al., [7]). However, given that tilapia populations may be susceptible to these two viruses due to its sequence resemblance to the TiLVs identified so far, further analyses are required to evaluate the clinical relevance of the two TiLV-like viruses and investigate their geographic distribution and virus-host interactions. Since there is currently only one approved virus species within the I Amnoonviridae i , no species demarcation criterion is available now, to the best of the author's knowledge, to determine whether the two isolates are qualified enough to constitute a new virus species or in fact represent a distinct lineage of TiLV based on the sequence identity values and host ranges.