Results
Title

A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid.

Authors

Kwon, Y.; Cha, J.; Chiang, J.; Tran, G.; Giaever, G.; Nislow, C.; Hur, J.‐S.; Kwak, Y.‐S.

Abstract

Aim To determine uncovered antifungal activity of lichen-derived compound, vulpinic acid, by using chemical-genetic analyses. Methods and Results Haploinsufficiency and homozygous-profiling assays were performed, revealing that strains lacking GLC7, MET4, RFC2, YAE1 and PRP18 were sensitive to three concentrations (12·5, 25 and 50% of inhibitory concentration) of vulpinic acid and independently validated. To verify inhibition of those genes, cell cycle analysis using flow cytometry was performed and relative expressions were measured. Under vulpinic acid-treated condition, cell cycle was arrested in S and G2/M phases and sensitive strains' relative expressions were significantly lower than the wild type yeast. Conclusions Vulpinic acid mainly affects cell cycle, glycogen metabolism, transcription and translation to fungi. Significance and Impact of the Study Although lichen-derived compounds are commercially valuable, few studies have determined their modes of action. This study used a chemogenomic approach to gain insight into the mechanisms of one of well-known lichen-derived compound, vulpinic acid.

Subjects

COMPOSITION of lichens; ANTIFUNGAL agents; CHEMOGENOMICS; PARMELIACEAE; PHENYLACETATES; BIOCHEMICAL mechanism of action; GLYCOGEN; CELL cycle

Publication

Journal of Applied Microbiology, 2016, Vol 121, Issue 6, p1580

ISSN

1364-5072

Publication type

Academic Journal

DOI

10.1111/jam.13300