Immunoradiometric assay for quantification of serum antibodies to dental plaque antigen in immunized dogs.

Ahlstedt, Staffan; Rylander, Harald

An immunoradiometric assay (IRMA) was used for quantifying dog serum antibodies to antigens from dental plaque collected, from full-grown dogs. The antigens were adsorbed onto the inner surface of plastic tubes and then incubated with dog-anti-plaque serum. 1251I-labelled anti-dog plasma-immunoglobulin was used for quantification of the specific antibodies. Four 10 months old Beagle dogs in excellent gingival health were immunized for 10 weeks with ultrasonicated dog dental plaque. The anibody levels in antisera sampled 6, 8, 10 and 11 weeks after the first antigen injection were 2 to 5 times as high as those recorded before the immunizing period. The variability of the assay as judged from the difference between duplicate samples was found to be 18 % ± 4 (p < 0.01) of the mean value and the variability between the same serum ran on different test occasions 13 % ± 7 (p < 0.01). The specificity of the antigen-antibody reaction in the immuno assay was tested by inhibition experiments. Pre-incubation of the antisera with dental plaque antigen significantly inhibited the antigen-antibody reaction in the IRMA, while bovine serum albumin! did not.

IMMUNOGLOBULINS; BLOOD plasma; SERUM; ANTIGENS; PROSPECTING; IMMUNITY

Journal of Periodontal Research, 1975, Vol 10, Issue 4, p224

0022-3484

Academic Journal

10.1111/j.1600-0765.1975.tb00029.x