Results
Title

Multiple drug-resistant gene 1 in children with steroid-sensitive nephrotic syndrome.

Authors

Funaki, Satoshi; Takahashi, Shori; Wada, Naohiro; Murakami, Hitohiko; Harada, Kensuke

Abstract

Background: A full dose of corticosteroid is required to induce complete remission (CR) in steroid-sensitive nephrotic syndrome (SSNS), unless it is possible to taper and discontinue along with the course after CR. But the mechanism of this change in steroid sensitivity remains unknown. P-glycoprotein (PGP) can eliminate given corticosteroids from cytoplasm, which results in corticosteroid resistance. Therefore, drug delivery was analyzed using real-time polymerase chain reaction (PCR) of multiple drug-resistant gene 1 ( MDR1; encoding PGP) mRNA expression. Methods: Fourteen patients with SSNS (male/female: 14/0; age: 1–23 years; mean 10.4 years) were enrolled in the study. MDR1 mRNA gene expression of peripheral blood nucleated cells (PBNC), before and after CR (19 sets of blood samples), was quantified using real-time PCR. Results: The MDR1 mRNA levels before CR were variable in each patient, but there was an apparent decrease in the MDR1 gene expression of PBNC after CR ( P < 0.003). Conclusion: PGP may play a role in the tapering of corticosteroids after CR in SSNS.

Subjects

DRUG resistance; SYNDROMES; STEROIDS; POLYMERASE chain reaction; ADRENOCORTICAL hormones

Publication

Pediatrics International, 2008, Vol 50, Issue 2, p159

ISSN

1328-8067

Publication type

Academic Journal

DOI

10.1111/j.1442-200X.2008.02541.x