The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots.

Tominaga-Wada, Rumi; Iwata, Mineko; Sugiyama, Junji; Kotake, Toshihisa; Ishida, Tetsuya; Yokoyama, Ryusuke; Nishitani, Kazuhiko; Okada, Kiyotaka; Wada, Takuji

Arabidopsis root hair formation is determined by the patterning genes CAPRICE ( CPC), GLABRA3 ( GL3), WEREWOLF ( WER) and GLABRA2 ( GL2), but little is known about the later changes in cell wall material during root hair formation. A combined Fourier-transform infrared microspectroscopy–principal components analysis (FTIR-PCA) method was used to detect subtle differences in the cell wall material between wild-type and root hair mutants in Arabidopsis. Among several root hair mutants, only the gl2 mutation affected root cell wall polysaccharides. Five of the 10 genes encoding cellulose synthase ( CESA1– 10) and 4 of 33 xyloglucan endotransglucosylase ( XTH1– 33) genes in Arabidopsis are expressed in the root, but only CESA5 and XTH17 were affected by the gl2 mutation. The L1-box sequence located in the promoter region of these genes was recognized by the GL2 protein. These results indicate that GL2 directly regulates cell wall-related gene expression during root development.

PLANT diseases; ARABIDOPSIS; GENES; CELL Walls & Membranes (Book); GENETIC mutation; ROOT development

Plant Journal, 2009, Vol 60, Issue 3, p564

0960-7412

Academic Journal

10.1111/j.1365-313X.2009.03976.x