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Title: Antisense Oligodeoxynucleotides for IL-2, c-myc and Transferrin Receptor Synchronize Mitogen-Activated Lymphocytes in the G1 Phase.
Authors: Kato, J.; Kohgo, Y.; Kondo, H.; Sasaki, K.; Niitsu, Y.
Abstract: In order to clarify the role and interrelationship of c-myc, interleukin-2 (IL-2), and transferrin receptor (TfR) expressions on PHA-stimulated lymphocyte proliferation, we examined the effects of antisense oligodeoxynucleotides directed against c-myc, TfR, and IL-2 mRNAs on DNA synthesis and cell-cycle phase. Exposure of PHA-stimulated lymphocytes to each antisense oligomer resulted in approximately 75-80% inhibition of DNA synthesis. TfR expression was not inhibited in PHA-stimulated lymphocytes by c-myc or IL-2 antisense oligonucleotides, suggesting that the expression of c-myc, TfR, and of IL-2 is regulated by an independent mechanism. All three antisense oligonucleotides for c-myc, TfR and IL-2 synchronized mitogen-activated lymphocytes to the G1 phase as assessed by morphologic blast formation and cell-cycle phase analysis.
Subjects: LYMPHOCYTES; INTERLEUKIN-2; CELL cycle; OLIGOMERS; MESSENGER RNA; OLIGONUCLEOTIDES
Publication: Scandinavian Journal of Immunology, 1994, Vol 39, Issue 5, p499
ISSN: 0300-9475
Publication type: Academic Journal
DOI: 10.1111/j.1365-3083.1994.tb03405.x

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Antisense Oligodeoxynucleotides for IL-2, c-myc and Transferrin Receptor Synchronize Mitogen-Activated Lymphocytes in the G1 Phase.