Functional expression of high-affinity receptor for immunoglobulin E on mast cells precedes that of tryptase during differentiation from human bone marrow-derived CD34 progenitors cultured in the presence of stem cell factor and interleukin-6.

Shimizu, Y.; Suga, T.; Maeno, T.; Aoki, F.; Tsukagoshi, H.; Kawata, T.; Sakai, K.; Narita, T.; Takahashi, T.; Ishikawa, S.; Morishita, Y.; Nakajima, T.; Hara, F.; Miura, T.; Kurabayashi, M.

CD34 progenitor cells develop into tryptase , CD117 mast cells when cultured in the presence of recombinant human stem cell factor (rhSCF). However, spontaneous IgE receptor (FCεRI) expression during human mast cell development is not well examined. Here, the expression and function of FCεRI in and on human bone marrow-derived mast cells (HBMMCs) during development were investigated. At 4 weeks of culture, predominant cells expressed high-affinity IgE receptor α chain (FCεRIα) on the cell surface determined by flow cytometry, but CD117 was less expressed. Immunocytochemistry with antitryptase mAB and anti-FCεRIα mAb revealed intracellular and surface expression of FCεRIα at 2 weeks of culture, but tryptase was less expressed. FCεRIα mRNA transcript preceded that of tryptase mRNA at 2 weeks of culture determined by real-time RT-PCR, and FCεRIα FCεRIβ, FCεRIγ, and tryptase mRNA increased along with differentiation. FCεRIα cross-link on HBMMC and 4-week-old mast cells/mast cell precursors induced the release of IL-5 and granulocyte macrophage-colony stimulating factor, which was enhanced by rhSCF. These data indicated that HBMMC constitutively and spontaneously expressed functional FC&psilon;RI subunits at the early stage of differentiation, probably because of the differences in the ability and functional property of progenitors.

IMMUNOGLOBULIN E; MAST cells; BONE marrow; CELL differentiation; IMMUNOHISTOCHEMISTRY; POLYMERASE chain reaction

Clinical & Experimental Allergy, 2004, Vol 34, Issue 6, p917

0954-7894

Academic Journal

10.1111/j.1365-2222.2004.01971.x