LECTIN-LIKE COMPOUNDS AND LECTIN RECEPTORS IN MARINE MICROALGAE: HEMAGGLUTINATION AND REACTIVITY WITH PURIFIED LECTINS.

Hori, Kanji; Ogata, Takehiko; Kamiya, Hisao; Mimuro, Mamoru

We detected lectin-like compounds and lectin receptors in microalgae by hemagglutination, competitive inhibition with sugers, and reactivity with lectins isolated from other sources,. Cell extracts from eight species of Dinophyceae and from one species each of Raphidophyceae and Bacillariophycene exhibited hemagglutination toward trypsinized rabbit erythrocytes. In addition, the culture media of the dinoflagellate Alexandrium cohorticula and the raphidophyte Chattonella antiqua displayed similar hemagglutination. These activities were not inhibited by any monosaccharides or oligosaccharides tested but were inhibited by some specific glycoproteins. This suggests that the active factors were lectin-like compounds. Upon exposing intact, healthy cells of 12 species of dinophyceae and one species each of Raphidophyceae, Cryptophyceae, Bacillarophyyceae, and Chlorophyceae to lectins isolated from either macroalgae or terrestrial plants, most species were adversely affected. The negative effects included one or more of the following impaired motility, disappearance of motility, agglutination, abnormal morphology, and cell rupture or lysis. Some species, even after freezing, thawing and washing with saline solution, still agglutinated with macroalgal or terrestrial plant lectins. This study suggests that lectins and carbohydrate-containing lectin receptors may commonly occur on the cell surfaces of various species of microalgae.

ALGAE; LECTINS; BLOOD agglutination; DINOFLAGELLATES; IMMUNOGLOBULINS; AGGLUTINATION

Journal of Phycology, 1996, Vol 32, Issue 5, p783

0022-3646

Academic Journal

10.1111/j.0022-3646.1996.00783.x