Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters.

Seifi, Soroush; Shahverdi, Abdolhossein; Rezaei Topraggaleh, Tohid; Sharafi, Mohsen; Esmaeili, Vahid; Choobineh, Hamid; Zamiri, Mohammad Javad; Habibi, Mohammad; Alizadeh, AliReza

The objective was to evaluate the effect of inclusion of 2.5% and 5% ovine serum, enriched with vitamin E (Vit E) and fish oil (FO), in human sperm freezing medium. Serum samples were prepared from sixteen rams (n = 4) feeding on a without supplemented diet, and diets supplemented with Vit E, FO and Vit E + FO. Semen samples, from 60 normozoospermic men, were frozen in: (I) a commercial freezing medium (SpermFreeze™; control medium), (II) the commercial freezing medium containing foetal bovine serum, (III) the commercial freezing medium + nonenriched serum (serum group), (IV) the commercial freezing medium + Vit E enriched serum (Vit E group), (V) the commercial freezing medium + FO enriched serum (FO group) and (VI) the commercial freezing medium + Vit E + FO enriched serum (Vit E + FO group). Sperm total and progressive motility, morphology, viability and plasma membrane integrity were significantly higher (p ≤.05) in Vit E and Vit E + FO groups compared with the control group. Mitochondrial membrane potential did not differ between treatments (p >.05). It was concluded that ovine serum enriched with vitamin E and vitamin E + FO improved the quality of human spermatozoa but enriched serum containing FO could not improve the sperm cryo‐injuries.

VITAMIN E; UNSATURATED fatty acids; SPERMATOZOA; MASS media; FISH oils

Andrologia, 2020, Vol 52, Issue 4, p1

0303-4569

Academic Journal

10.1111/and.13541