This study aims to assess education on the use of mineral trioxide aggregate ( MTA) and Biodentine among members of the Australian Society of Endodontology ( ASE), a society of specialist endodontists ( ED) and general dentists with an interest in endodontics ( GD). The study also aims to compare the procedural preferences relating to perforation repair, apical barrier, root-end filling and regenerative endodontics. A structured online questionnaire was used, which sought details of the education in the use of MTA and the procedural steps involved in perforation repair, apical barrier, root-end filling and regenerative endodontics. Fisher's exact test was performed to compare the GD with ED. Responses were received from 208 out of 499 ASE members. Some 40% of the total respondents were ED. Almost all ED (98.8%) and some GD (39.8%) used MTA for perforation repairs. Likewise, almost all ED (96.3%) and some GD (42.7%) used MTA for apical barrier procedures. Lack of experience was more of a barrier to its use for GD (48.7%) than its high cost (31.6%). Few members used Biodentine. Significant differences exist in how MTA is used between GD and ED. Experience in handling MTA is a larger barrier to its widespread use in endodontics than its cost.