The purpose of this study was to evaluate the tooth discolouration potential of two new calcium-silicate-based sealers (mineral trioxide aggregate ( MTA) Fillapex and iRoot SP). Sixty premolars were resected below the coronal third of the root. Pulp chamber was instrumented and irrigated via the apical access. Samples were randomly assigned into groups A ( MTA Fillapex), B ( AH plus), C (iRoot SP) and D (unfilled as control). Colour assessment was performed at baseline, 1st, 3rd and 6th months with an intraoral spectrophotometer. Analysis of variance with additional multiple comparisons of post hoc were used for statistical analysis. All sealers caused perceptible tooth discolouration and there was no statistically significant difference between them. Discolouration progressed within the first 3 months and then decreased until the 6th month.