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Title: Identification and molecular characterisation of lactobacilli isolated from traditional Koopeh cheese.
Authors: Hassanzadazar, Hassan; Mardani, Karim; Yousefi, Mohammad; Ehsani, Ali
Abstract: This study was undertaken in order to phenotype and genotype wild-type lactic acid bacteria isolated from Koopeh cheese of West Azerbaijan. Lactic acid bacteria ( LAB) isolates were identified by polymerase chain reaction ( PCR) and restriction fragment length polymorphism ( RFLP) and confirmed by phylogenetic analysis. Genotyping based on phylogenetic analysis of 16s rDNA gene revealed that LAB isolated from Koopeh cheese were mostly Lactobacillus plantarum as well as other species including Lactobacillus brevis, Entrococcus faecium and Enterococcus durans. It was concluded that a combined phenotypic and genotypic method could be used as a reliable technique for the identification and differentiation of LAB from dairy products.
Subjects: LACTOBACILLUS; CHEESE microbiology; LACTIC acid bacteria; PHYLOGENY; PHENOTYPES; GENOTYPES
Publication: International Journal of Dairy Technology, 2017, Vol 70, Issue 4, p556
ISSN: 1364-727X
Publication type: Academic Journal
DOI: 10.1111/1471-0307.12396

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Identification and molecular characterisation of lactobacilli isolated from traditional Koopeh cheese.