The results of the field and laboratory studies of radiation and environmental state at the specific area of Irtysh River adjacent to the Semipalatinsk Test Site are provided. It was found that the radiation situation in this area is normal: equivalent dose of γ-radiation 5 (0.11-0.13) μSv h-1. Determination of radionuclide composition of soil, bottom sediment and water samples was performed by the methods of instrumental γ-spectrometry, radiochemical analysis and the liquid scintillation β-spectrometry. It was found that concentrations of the studied natural and artificial radionuclides in these objects are very low; no contamination with radionuclides was detected in this segment of Irtysh River. The article provides the results of elemental composition determination for samples of soil and bottom sediment (by X-ray fluorescence method) and water samples (by inductively coupled plasma mass spectrometry method). It is shown that the content of some elements (Li, Be, B, V, Cu, Sr, Mo) in the water of Irtysh River increases downstream. The additional studies are required to explain this peculiarity.