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- Title
Preparation and evaluation of decellularized epineurium as an anti-adhesive biofilm in peripheral nerve repair.
- Authors
Li, Xiao; Tao, Meihan; Quan, Liang; Zhang, Hengtong; Xin, Yuan; Wu, Xixi; Fang, Xinyu; Fan, Jun; Tian, Xiaohong; Wang, Xiaohong; Wen, Lili; Yu, Tianhao; Ao, Qiang
- Abstract
Following peripheral nerve anastomosis, the anastomotic site is prone to adhesions with surrounding tissues, consequently impacting the effectiveness of nerve repair. This study explores the development and efficacy of a decellularized epineurium as an anti-adhesive biofilm in peripheral nerve repair. Firstly, the entire epineurium was extracted from fresh porcine sciatic nerves, followed by a decellularization process. The decellularization efficiency was then thoroughly assessed. Subsequently, the decellularized epineurium underwent proteomic analysis to determine the remaining bioactive components. To ensure biosafety, the decellularized epineurium underwent cytotoxicity assays, hemolysis tests, cell affinity assays, and assessments of the immune response following subcutaneous implantation. Finally, the functionality of the biofilm was determined using a sciatic nerve transection and anastomosis model in rats. The result indicated that the decellularization process effectively removed cellular components from the epineurium while preserving a number of bioactive molecules, and this decellularized epineurium was effective in preventing adhesion while promoting nerve repairment and functional recovery. In conclusion, the decellularized epineurium represents a novel and promising anti-adhesion biofilm for enhancing surgical outcomes of peripheral nerve repair.
- Subjects
SCIATIC nerve; PERIPHERAL nervous system; LABORATORY rats; CYTOTOXINS; CELL anatomy
- Publication
Regenerative Biomaterials, 2024, Vol 11, p1
- ISSN
2056-3418
- Publication type
Academic Journal
- DOI
10.1093/rb/rbae054