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- Title
A New 9-Lipoxygenase cDNA from Developing Rice Seeds.
- Authors
Kouichi Mizuno; Toshii Iida; Atsushi Takano; Mineyuki Yokoyama; Tatsuhito Fujimura
- Abstract
We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.
- Subjects
LIPOXYGENASES; DNA; GERMINATION; RICE
- Publication
Plant & Cell Physiology, 2003, Vol 44, Issue 11, p1168
- ISSN
0032-0781
- Publication type
Academic Journal
- DOI
10.1093/pcp/pcg142