EBSCO Logo
Connecting you to content on EBSCOhost
Results
Title

Pharmacological potential of 4-dimethylamino chalcone against acute and neuropathic pain in mice.

Authors

Marchon, Isabela Souza dos Santos; Melo, Evelynn Dalila do Nascimento; Botinhão, Mirella da Costa; Pires, Greice Nascimento; Reis, João Vitor Rocha; de Souza, Rodrigo Octavio Mendonça Alves; Leal, Ivana Correa Ramos; Bonavita, André Gustavo Calvano; Mendonça, Henrique Rocha; Muzitano, Michelle Frazão; da Silva, Leandro Louback; do Carmo, Paula Lima; Raimundo, Juliana Montani

Abstract

Objectives: This work investigated the acute antinociceptive effect of a synthetic chalcone, 4-dimethylamino chalcone (DMAC), as well as its effects on vincristine-induced peripheral neuropathy (VIPN) in mice. Methods: The inhibitory activity of myeloperoxidase was assessed by measuring HOCl formation. Formalin and hot plate tests were used to study the acute antinociceptive effect of DMAC. VIPN was induced through the administration of vincristine sulphate (0.1 mg/kg, i.p., 14 days). Then, DMSO, DMAC (10 or 30 mg/kg; i.p.), or pregabalin (10 mg/kg, i.p.) were administered for 14 consecutive days. Thermal hyperalgesia and mechanical allodynia were evaluated before and after VIPN induction and on days 1, 3, 7, and 14 of treatment. Neurodegeneration and neuroinflammation were assessed through immunohistochemistry for NF200, iNOS, and arginase-1 within the sciatic nerve. Key findings: DMAC inhibited myeloperoxidase activity in vitro and presented an acute antinociceptive effect in both formalin and hot plate tests, with the involvement of muscarinic and opioid receptors. Treatment with 30 mg/kg of DMAC significantly attenuated thermal hyperalgesia and mechanical allodynia and prevented macrophage proinflammatory polarisation in VIPN mice. Conclusions: Our results show that DMAC, acting through different mechanisms, effectively attenuates VIPN.

Subjects

MUSCARINIC receptors; OPIOID receptors; NEURALGIA; SCIATIC nerve; MYELOPEROXIDASE; CHALCONE

Publication

Journal of Pharmacy & Pharmacology, 2024, Vol 76, Issue 8, p983

ISSN

0022-3573

Publication type

Academic Journal

DOI

10.1093/jpp/rgae057

EBSCO Connect | Privacy policy | Terms of use | Copyright | Manage my cookies
Journals | Subjects | Sitemap
© 2025 EBSCO Industries, Inc. All rights reserved