Fine structure of Aldrovanda vesiculosa L: the peculiar lifestyle of an aquatic carnivorous plant elucidated by electron microscopy using cryo-techniques.

Atsuzawa, Kimie; Kanaizumi, Daiki; Ajisaka, Mizuki; Kamada, Tasuku; Sakamoto, Kimie; Matsushima, Hisashi; Kaneko, Yasuko

The aquatic carnivorous plant Aldrovanda vesiculosa L. is critically endangered worldwide; its peculiar lifestyle raises many questions and poses problems both intriguing on their own and relevant to conservation. While establishing a culture system for its propagation and restoring its natural habitat in Hozoji pond in Saitama, Japan, we conducted ultrastructural observations to examine the various aspects of Aldrovanda' s way of life. Electron microscopic observation in combination with cryo-techniques produced novel information which could not be obtained by other methods. Some of the results are: phosphorous is stored in petiole cells of turions during winter; mucilaginous guides are provided for pollen tubes in parietal placental ovaries; storage of potassium in the vicinity of the midrib of carnivorous leaves may contribute to the rapid closing of the carnivorous leaves; dynamic sequential changes of the ultrastructure of digestive glands are involved in the synthesis and secretion of digestive enzymes, including protease and acid phosphatase. These results should contribute significantly to our understanding of Aldrovanda and the detailed mechanisms of its life.

CARNIVOROUS plants; AQUATIC plants; ELECTRON microscopy; ACID phosphatase; POLLEN tube; DIGESTIVE enzymes

Microscopy, 2020, Vol 69, Issue 4, p214

2050-5698

Academic Journal

10.1093/jmicro/dfaa019