Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using ¹³C-glucose.

Rogals, Monique J; Yang, Jeong-Yeh; Williams, Robert V; Moremen, Kelley W; Amster, I Jonathan; Prestegard, James H

Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N. This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used. However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used. We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1. We demonstrate an ability to detect partially occupied N -glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence. The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.

NUCLEAR magnetic resonance; GLYCANS; RADIOLABELING; GLYCOPROTEINS; AMINO acid sequence; BACTERIAL cultures

Glycobiology, 2021, Vol 31, Issue 4, p425

0959-6658

Academic Journal

10.1093/glycob/cwaa071