P599 Previous exercise training reduces arterial pressure, adipose tissue and improves oxidative stress in a model of menopause.

Palma, R K; Paixao, C; Shimojo, GL; Bernardes, N; Barboza, CA; Lopes, AB; De Angelis, K; Malfitano, C

Purpose: Exercise training is an important intervention non-pharmacological for attenuation of metabolic and cardiovascular dysfunctions triggered by the advent of menopause. However, it is not known whether previous exercise training intervention alters the physiological and medical complications of these. Thereby, the purpose of the present study was to investigate the effects of previous exercise training (PT) on metabolic and hemodynamic parameters and oxidative stress in ovariectomized rats.Methods: Female Wistar rats were divided into 2 groups (n=6 each): sedentary ovariectomized (SO) and previously trained ovariectomized (PTO). The PT was performed on a treadmill (5days per week/12week), in which were 4 weeks before ovariectomy and more 8 weeks after ovariectomy. Arterial pressure (AP) and heart rate (HR) were directly recorded. The parametrial and subcutaneous adipose tissues were removed and weighed after euthanasia. Oxidative stress was evaluated in left ventricle tissue by lipid peroxidation by chemiluminescence (QL), catalase (CAT) and superoxide dismutase (SOD) determinations.Results: The previous exercise training induced reduction in AP (PTO: 115.55 ± 0.7 vs. SO: 120.31 ± 0.8 mmHg) and resting bradycardia (PTO: 331 ± 6 vs. SO: 366 ± 11 bpm). Body weight was not different between groups (PTO: 336 ± 4 vs. SO: 340 ± 14g). However, adipose tissue weight was reduced in the PTO group, both parametrial (1.8 ± 0.1g) and subcutaneous (2.26 ± 0.08g) when compared to the SO group (2.77 ± 0:16; 2.81 ± 0.07g, respectively). Lipid peroxidation was reduced in trained group in relation to sedentary group (1069 ± 110 vs. 2935±231 cps/mg protein) and the antioxidant enzyme activities, CAT (PTO: 0.87±0.06 vs. SO: 0.51±0.07 nmol/mg protein) and SOD (PCO: 24.57±1.35 vs. OS: 18.18±1.16 USOD/mg protein), were higher in trained group.Conclusion: The previous exercise training induced reduction in blood pressure and resting bradycardia, associated with reduced adipose tissue and improved oxidative profile on cardiac tissue in ovariectomized rats. These dates suggest a positive role of this non-pharmacological approach to prevent disarrangements triggered by ovarian hormones deprivation.

EXERCISE therapy; ADIPOSE tissues; OXIDATIVE stress; MENOPAUSE; HEMODYNAMIC monitoring; LABORATORY rats

Cardiovascular Research, 2014, Vol 103, Issue suppl_1, pS108

0008-6363

Academic Journal

10.1093/cvr/cvu098.28