Preparation and Evaluation of Poly(N-isopropylacrylamide-co-hexanediol diacrylate-co-ethylene dimethacrylate) Monolithic Column and its Applications in the Separation of Small Molecules.

Yamin Ma; Haiyan Liu; Aile Wei; Junxiao Qin; Shiqi Zhang; Wen Zhang; Ligai Bai

Poly(N-isopropylacrylamide-co-hexanediol diacrylate-co-ethylene dimethacrylate) [poly(NIPAAmco- HDDA-co-EDMA)] monolithic column was prepared via in situ polymerization reaction. In order to investigate the porous properties of the monoliths prepared, the morphology was characterized by the scanning electron microscopy; the chemical group of the monolithic column was confirmed by a Fourier transform infrared spectroscopy method. The surface area was 39.1m²/g by the nitrogen adsorption-desorption experiment. With methanol as the mobile phase, the permeability of the monolithic column was calculated as 3.2330 × 10-14m². Then it was used as the stationary phase of high performance liquid chromatography. The results indicted that poly(NIPAAm-co- HDDA-co-EDMA) monolithic column was good to separate small molecules by controlling the temperature. Column efficiency for p-chloronitrobenzene was 4,680 plates/m. Repeatability was defined by determining run-to-run and column-to-column variation of the retention times of aromatic compounds, expressed as relative standard deviation (RSD = standard deviation/mean × 100%), and the values were <0.58% and 3.1%, respectively.

ACRYLAMIDE; MONOLITHIC reactors; ETHYLENE compounds; CHEMICAL reactions; SCANNING electron microscopy

Journal of Chromatographic Science, 2016, Vol 54, Issue 10, p1727

0021-9665

Academic Journal

10.1093/chromsci/bmw129