GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 functions upstream of reactive carbonyl species production in Arabidopsis guard-cell abscisic acid signaling.

Shaiek, Oumayma; Yin, Huifei; Uesako, Nodoka; Islam, Md Moshiul; Rhaman, Mohammad Saidur; Nakamura, Toshiyuki; Nakamura, Yoshimasa; Munemasa, Shintaro; Mano, Jun'ichi; Murata, Yoshiyuki

GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1), a leucine-rich repeat receptor-like kinase, is involved in abscisic acid (ABA)-induced stomatal closure. We investigated the role of GHR1 in reactive oxygen species (ROS) signaling for ABA-induced stomatal closure. ABA induced ROS production in wild type (WT) and the ghr1 of Arabidopsis thaliana. Hydrogen peroxide induced stomatal closure, accompanying the generation of acrolein in guard cells. The reactive carbonyl species (RCS) scavengers inhibited the ABA- and H2O2-induced stomatal closure in WT. In the ghr1 , H2O2 failed to induce acrolein production and stomatal closure while RCS induced stomatal closure. Thus, GHR1 functions downstream of ROS and is required for the generation of RCS in guard-cell ABA signaling. In the ghr1 , Ca2 induced stomatal closure but RCS did not activate I Ca channels. The GHR1 may be also involved in a Ca2 -independent pathway for ABA-induced stomatal closure.

GUARD cells (Plant anatomy); REACTIVE oxygen species; ARABIDOPSIS thaliana; HYDROGEN peroxide; ABSCISIC acid; FREE radical scavengers

Bioscience, Biotechnology & Biochemistry, 2024, Vol 88, Issue 12, p1403

0916-8451

Academic Journal

10.1093/bbb/zbae135