Results
Title

Production of a new triterpenoid disaccharide saponin from sequential glycosylation of ganoderic acid A by 2 Bacillus glycosyltransferases.

Authors

Chang, Te-Sheng; Chiang, Chien-Min; Wu, Jiumn-Yih; Tsai, Yu-Li; Ting, Huei-Ju

Abstract

Ganoderic acid A (GAA) is a lanostane-type triterpenoid, isolated from medicinal fungus Ganoderma lucidum , and possesses multiple bioactivities. In the present study, GAA was sequentially biotransformed by 2 recently discovered Bacillus glycosyltransferases (GT), BtGT_16345 and BsGT110, and the final product was purified and identified as a new compound, GAA-15,26- O -β-diglucoside, which showed 1024-fold aqueous solubility than GAA.

Subjects

SAPONINS; GLYCOSYLTRANSFERASES; BACILLUS (Bacteria); DISACCHARIDES; TRITERPENOIDS; GANODERMA lucidum; GLYCOSYLATION

Publication

Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 3, p687

ISSN

0916-8451

Publication type

Academic Journal

DOI

10.1093/bbb/zbaa055