Results
Title

A specialized tyrosine-based endocytosis signal in MR1 controls antigen presentation to MAIT cells.

Authors

Hui Jing Lim; Wubben, Jacinta M.; Garcia, Cristian Pinero; Cruz-Gomez, Sebastian; Deng, Jieru; Mak, Jeffrey Y. W.; Hachani, Abderrahman; Anderson, Regan J.; Painter, Gavin F.; Goyette, Jesse; Amarasinghe, Shanika L.; Ritchie, Matthew E.; Roquilly, Antoine; Fairlie, David P.; Gaus, Katharina; Rossjohn, Jamie; Villadangos, Jose A.; McWilliam, Hamish E. G.

Abstract

MR1 is a highly conserved microbial immune-detection system in mammals. It captures vitamin B-related metabolite antigens from diverse microbes and presents them at the cell surface to stimulate MR1-restricted lymphocytes including mucosalassociated invariant T (MAIT) cells. MR1 presentation and MAIT cell recognition mediate homeostasis through host defense and tissue repair. The cellular mechanisms regulating MR1 cell surface expression are critical to its function and MAIT cell recognition, yet they are poorly defined. Here, we report that human MR1 is equipped with a tyrosine-based motif in its cytoplasmic domain that mediates low affinity binding with the endocytic adaptor protein 2 (AP2) complex. This interaction controls the kinetics of MR1 internalization from the cell surface and minimizes recycling. We propose MR1 uses AP2 endocytosis to define the duration of antigen presentation toMAIT cells and the detection of a microbial metabolic signature by the immune system.

Subjects

ANTIGEN presentation; CELLULAR recognition; ENDOCYTOSIS; ADAPTOR proteins; CELL physiology; MICROBIAL cells

Publication

Journal of Cell Biology, 2022, Vol 221, Issue 12, p1

ISSN

0021-9525

Publication type

Academic Journal

DOI

10.1083/jcb.202110125