Works matching DE "AFFINITY chromatography"

Results: 1534

Cell-Penetrating Peptide Based on Myosin Phosphatase Target Subunit Sequence Mediates Myosin Phosphatase Activity.

Published in:

Biomolecules (2218-273X), 2025, v. 15, n. 5, p. 705, doi. 10.3390/biom15050705

By:

Kiss, Andrea;
Mahfood, Mohamad;
Bodogán, Zsófia;
Kónya, Zoltán;
Bécsi, Bálint;
Erdődi, Ferenc

Publication type:

Article

A Molecular Form of Russian Sturgeon Gonadotropin without Affinity for Concanavalin A.

Published in:

Doklady Biochemistry & Biophysics, 2001, v. 376, n. 1-6, p. 13, doi. 10.1023/A:1018887723597

By:

Zenkevich, G. A.;
Vose, V. A.;
Butsena, A. Ya.

Publication type:

Article

Biochemical characterization of an esterase from Thermobifida fusca YX with acetyl xylan esterase activity.

Published in:

Molecular Biology Reports, 2024, v. 51, n. 1, p. 1, doi. 10.1007/s11033-024-09601-7

By:

da Silva, Adriana S.;
Adriani, Patricia P.;
de Oliveira, Gabriel S.;
Rocha, Adriana Rios Lopes;
Perpétuo, Elen A.;
Dias, Marcio V. B.;
Chambergo, Felipe S.

Publication type:

Article

Expression of soluble moloney murine leukemia virus-reverse transcriptase in Escherichia coli BL21 star (DE3) using autoinduction system.

Published in:

Molecular Biology Reports, 2024, v. 51, n. 1, p. 1, doi. 10.1007/s11033-024-09583-6

By:

Handayani, Christina Vivid;
Laksmi, Fina Amreta;
Andriani, Ade;
Nuryana, Isa;
Mubarik, Nisa Rachmania;
Agustriana, Eva;
Dewi, Kartika Sari;
Purnawan, Awan

Publication type:

Article

Bacterial over-production of the functionally active human SLC38A2 (SNAT2) exploiting the mistic tag: a cheap and fast tool for testing ligands.

Published in:

Molecular Biology Reports, 2024, v. 51, n. 1, p. 1, doi. 10.1007/s11033-023-08976-3

By:

Galluccio, Michele;
Tripicchio, Martina;
Console, Lara;
Indiveri, Cesare

Publication type:

Article

Proteomic analysis of copper-binding proteins in excess copper-stressed rice roots by immobilized metal affinity chromatography and two-dimensional electrophoresis.

Published in:

BioMetals, 2014, v. 27, n. 2, p. 265, doi. 10.1007/s10534-014-9707-x

By:

Song, Yufeng;
Zhang, Hongxiao;
Chen, Chen;
Wang, Guiping;
Zhuang, Kai;
Cui, Jin;
Shen, Zhenguo

Publication type:

Article

In silico prediction of drug-binding strengths to human serum albumin.

Published in:

Medicinal Research Reviews, 2003, v. 23, n. 3, p. 275, doi. 10.1002/med.10039

By:

Gonzalo Colmenarejo

Publication type:

Article

High yield expression and efficient purification of deuterated human protein galectin-2.

Published in:

Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2012, v. 90, n. 3, p. 563, doi. 10.1016/j.fbp.2011.12.004

By:

Xiaojing Chen;
Wilde, Karyn L.;
Hui Wang;
Lake, Vanessa;
Holden, Peter J.;
Middelberga, Anton P. J.;
He, Lizhong;
Duff, Anthony P.

Publication type:

Article

Basidiomycete Clitocybe nebularis is rich in lectins with insecticidal activities.

Published in:

Applied Microbiology & Biotechnology, 2011, v. 91, n. 4, p. 1141, doi. 10.1007/s00253-011-3236-0

By:

Pohleven, Jure;
Brzin, Jože;
Vrabec, Lara;
Leonardi, Adrijana;
Čokl, Andrej;
Štrukelj, Borut;
Kos, Janko;
Sabotič, Jerica

Publication type:

Article

Production and characterization of LEA29Y, a variant of cytotoxic T-lymphocyte antigen 4-immunoglobulin, in Pichia pastoris.

Published in:

Applied Microbiology & Biotechnology, 2011, v. 91, n. 3, p. 543, doi. 10.1007/s00253-011-3277-4

By:

Lin Wan;
Shengyun Zhu;
Yingying Li;
Shan Liu;
Hao Yang;
Shengfu Li;
Youping Li;
Jingqiu Cheng;
Xiaofeng Lu

Publication type:

Article

A thermostable feruloyl-esterase from the hemicellulolytic bacterium Thermobacillus xylanilyticus releases phenolic acids from non-pretreated plant cell walls.

Published in:

Applied Microbiology & Biotechnology, 2011, v. 90, n. 2, p. 541, doi. 10.1007/s00253-011-3103-z

By:

Rakotoarivonina, Harivony;
Hermant, Beatrice;
Chabbert, Brigitte;
Touzel, Jean-Pierre;
Remond, Caroline

Publication type:

Article

Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.

Published in:

Applied Microbiology & Biotechnology, 2010, v. 88, n. 2, p. 529, doi. 10.1007/s00253-010-2732-y

By:

Stammen, Simon;
Schuller, Franziska;
Dietrich, Sylvia;
Gamer, Martin;
Biedendieck, Rebekka;
Jahn, Dieter

Publication type:

Article

Soluble expression and purification of the anthrax protective antigen in E. coli and identification of a novel dominant-negative mutant N435C.

Published in:

Applied Microbiology & Biotechnology, 2010, v. 87, n. 2, p. 609, doi. 10.1007/s00253-010-2495-5

By:

Gaobing Wu;
Chunfang Feng;
Yuzhi Hong;
Aizhen Guo;
Sha Cao;
Junli Dong;
Ling Lin;
Ziduo Liu

Publication type:

Article

Isolation of a thermostable legume chitinase and study on the antifungal activity.

Published in:

Applied Microbiology & Biotechnology, 2009, v. 85, n. 2, p. 313, doi. 10.1007/s00253-009-2074-9

By:

Wang, Shaoyun;
Shao, Biao;
Fu, Hong;
Rao, Pingfan

Publication type:

Article

An extracellular polyhydroxybutyrate depolymerase in Thermus thermophilus HB8.

Published in:

Applied Microbiology & Biotechnology, 2009, v. 83, n. 4, p. 659, doi. 10.1007/s00253-008-1842-2

By:

Papaneophytou, Christos P.;
Pantazaki, Anastasia A.;
Kyriakidis, Dimitrios A.

Publication type:

Article

Large-scale production of biologically active human keratinocyte growth factor-2.

Published in:

Applied Microbiology & Biotechnology, 2009, v. 82, n. 3, p. 439, doi. 10.1007/s00253-008-1782-x

By:

Wu, Xiaoping;
Tian, Haishan;
Huang, Yadong;
Wu, Sixian;
Liu, Xiaoju;
Wang, Cong;
Wang, Xiaojie;
Huang, Zhifeng;
Xiao, Jian;
Feng, Wenke;
Li, Xiaokun

Publication type:

Article

Isolation and biochemical characterization of a novel leguminous defense peptide with antifungal and antiproliferative potency.

Published in:

Applied Microbiology & Biotechnology, 2009, v. 82, n. 1, p. 79, doi. 10.1007/s00253-008-1729-2

By:

Wang, Shaoyun;
Rao, Pingfan;
Ye, Xiuyun

Publication type:

Article

Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 81, n. 4, p. 721, doi. 10.1007/s00253-008-1716-7

By:

Wardenga, Rainer;
Hollmann, Frank;
Thum, Oliver;
Bornscheuer, Uwe

Publication type:

Article

High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 81, n. 2, p. 311, doi. 10.1007/s00253-008-1655-3

By:

Ye, Tingmei;
Lin, Zhihua;
Lei, Huanzong

Publication type:

Article

Functional analysis of the Burkholderia cenocepacia J2315 BceA<sub>J</sub> protein with phosphomannose isomerase and GDP- d-mannose pyrophosphorylase activities.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 80, n. 6, p. 1015, doi. 10.1007/s00253-008-1612-1

By:

Sousa, Sílvia;
Moreira, Leonilde;
Leitão, Jorge

Publication type:

Article

High-level expression of his-tagged clostridial collagenase in Clostridium perfringens.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 80, n. 4, p. 627, doi. 10.1007/s00253-008-1592-1

By:

Tamai, Eiji;
Miyata, Shigeru;
Tanaka, Hiroaki;
Nariya, Hirofumi;
Suzuki, Motoo;
Matsushita, Osamu;
Hatano, Naoya;
Okabe, Akinobu

Publication type:

Article

Genome mining of cyanide-degrading nitrilases from filamentous fungi.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 80, n. 3, p. 427, doi. 10.1007/s00253-008-1559-2

By:

Basile, Lacy;
Willson, Richard;
Sewell, B.;
Benedik, Michael

Publication type:

Article

Purification and characterization of organic solvent stable protease from Bacillus licheniformis RSP-09-37.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 79, n. 3, p. 399, doi. 10.1007/s00253-008-1429-y

By:

Sareen, Ritu;
Mishra, Prashant

Publication type:

Article

Low-resolution structure, oligomerization and its role on the enzymatic activity of a sucrose-6-phosphate hydrolase from Bacillus licheniformis.

Published in:

Amino Acids, 2019, v. 51, n. 4, p. 599, doi. 10.1007/s00726-018-02690-2

By:

Mera, Alain;
de Lima, Mariana Zuliani Theodoro;
Bernardes, Amanda;
Garcia, Wanius;
Muniz, João Renato Carvalho

Publication type:

Article

Purification of angiotensin I-converting enzyme (ACE) inhibitory peptides from casein hydrolysate by IMAC-Ni.

Published in:

Amino Acids, 2017, v. 49, n. 10, p. 1787, doi. 10.1007/s00726-017-2475-5

By:

Wu, Shanguang;
Feng, Xuezhen;
Lu, Yuan;
Lu, Yuting;
Liu, Saisai;
Tian, Yuhong

Publication type:

Article

Combinatorial peptide libraries to overcome the classical affinity-enrichment methods in proteomics.

Published in:

Amino Acids, 2013, v. 45, n. 2, p. 219, doi. 10.1007/s00726-013-1505-1

By:

Righetti, Pier;
Boschetti, Egisto

Publication type:

Article

Enrichment techniques employed in phosphoproteomics.

Published in:

Amino Acids, 2012, v. 43, n. 3, p. 1025, doi. 10.1007/s00726-011-1111-z

By:

Fíla, Jan;
Honys, David

Publication type:

Article

Investigations on the activation of recombinant microbial pro-transglutaminase: in contrast to proteinase K, dispase removes the histidine-tag.

Published in:

Amino Acids, 2012, v. 42, n. 2/3, p. 997, doi. 10.1007/s00726-011-1016-x

By:

Sommer, Christian;
Hertel, Thomas;
Schmelzer, Christian;
Pietzsch, Markus

Publication type:

Article

Towards the analysis of protein species: an overview about strategies and methods.

Published in:

2011

By:

Jungblut, Peter;
Schlüter, Hartmut

Publication type:

Editorial

The function of BK channels extracted and purified within SMALPs.

Published in:

Biochemical Journal, 2022, v. 479, n. 15, p. 1609, doi. 10.1042/BCJ20210628

By:

Patel, Jaimin H.;
Pollock, Naomi L.;
Maher, Jacqueline;
Rothnie, Alice J.;
Allen, Marcus C.

Publication type:

Article

sfinx: an R package for the elimination of false positives from affinity purification-mass spectrometry datasets.

Published in:

Bioinformatics, 2017, v. 33, n. 12, p. 1902, doi. 10.1093/bioinformatics/btx076

By:

Titeca, Kevin;
Meysman, Pieter;
Laukens, Kris;
Martens, Lennart;
Tavernier, Jan;
Eyckerman, Sven

Publication type:

Article

Clustering by soft-constraint affinity propagation: applications to gene-expression data.

Published in:

Bioinformatics, 2007, v. 23, n. 20, p. 2708, doi. 10.1093/bioinformatics/btm414

By:

Leone, Michele;
Sumedha;
Weigt, Martin

Publication type:

Article

Metal-binding protein: Metallothionein.

Published in:

International Journal of Medical Biochemistry, 2023, v. 6, n. 1, p. 57, doi. 10.14744/ijmb.2022.94834

By:

Marikar, Faiz;
Hua Zi-Chun

Publication type:

Article

ISOLATION AND CHARACTERIZATION OF A PANCREATIC AMYLASE INHIBITOR.

Published in:

Journal of Chemistry & Technologies, 2022, v. 30, n. 4, p. 558, doi. 10.15421/jchemtech.v30i4.263201

By:

Krusir, Galyna V.;
Hugi, Christoph;
Breitenmoser, Lena;
Pylypenko, Liudmyla N.;
Sevastyanova, Elena V.;
Mazurenko, Kseniia I.

Publication type:

Article

Aggregation-Dispersion Chromatography: Application of Elastin-like Polypeptides.

Published in:

Separations (2297-8739), 2024, v. 11, n. 12, p. 335, doi. 10.3390/separations11120335

By:

Shin, Han Bin;
Chae, Young Kee

Publication type:

Article

Optimization of the Preparation of Hydrophilic Poly(DHPMA-co-MBA) Monolithic Capillary Columns: A New Support for Affinity Chromatography.

Published in:

Separations (2297-8739), 2023, v. 10, n. 8, p. 437, doi. 10.3390/separations10080437

By:

Gil, Julie;
Passalacqua, Gaëtan;
Deloche, Adrien;
Vidal, François-Xavier;
Dugas, Vincent;
Demesmay, Claire

Publication type:

Article

An Overview of Herbal Nutraceuticals, Their Extraction, Formulation, Therapeutic Effects and Potential Toxicity.

Published in:

Separations (2297-8739), 2023, v. 10, n. 3, p. 177, doi. 10.3390/separations10030177

By:

Bommakanti, Vaishnavi;
Puthenparambil Ajikumar, Amruthamol;
Sivi, Chelssa Maria;
Prakash, Geethika;
Mundanat, Anjaly Shanker;
Ahmad, Faraz;
Haque, Shafiul;
Prieto, Miguel Angel;
Rana, Sandeep Singh

Publication type:

Article

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents.

Published in:

Separations (2297-8739), 2023, v. 10, n. 1, p. 36, doi. 10.3390/separations10010036

By:

Silva, Josiane F. da;
Lima, Clara M. G.;
da Silva, Débora L.;
do Nascimento, Ivonea S.;
Rodrigues, Sarah de O.;
Gonçalves, Letícia A.;
Santana, Renata F.;
Khalid, Waseem;
Verruck, Silvani;
Emran, Talha Bin;
de Menezes, Irwin R. A.;
Coutinho, Henrique D. M.;
Khandaker, Mayeen U.;
Faruque, Mohammad R. I.;
Fontan, Rafael da C. I.

Publication type:

Article

Development of a Methodology to Adapt an Equilibrium Buffer/Wash Applied to the Purification of hGPN2 Protein Expressed in Escherichia coli Using an IMAC Immobilized Metal Affinity Chromatography System.

Published in:

Separations (2297-8739), 2022, v. 9, n. 7, p. N.PAG, doi. 10.3390/separations9070164

By:

Juárez-Lucero, Jorge;
Guevara-Villa, María del Rayo;
Sánchez-Sánchez, Anabel;
Díaz-Hernández, Raquel;
Altamirano-Robles, Leopoldo

Publication type:

Article

重组磷脂酶 D Sp-TLI146 的发掘及其在 DHA 磷脂酰单甘油酯的合成中的应用.

Published in:

Food Research & Development, 2024, v. 45, n. 21, p. 187, doi. 10.12161/j.issn.1005‐6521.2024.21.024

By:

贺晨曦;
陈曦;
刁汝静;
孙建安;
毛相朝

Publication type:

Article

CRISPR-Cas12a 检测牛奶中大肠杆菌方法的建立与评价.

Published in:

Food Research & Development, 2023, v. 44, n. 18, p. 179, doi. 10.12161/j.issn.1005-6521.2023.18.024

By:

冀霞;
代绍密;
余若菁;
黄冰冰;
刘家铭;
李玮玮

Publication type:

Article

镰刀菌 Fo47 来源木聚糖酶的异源表达、酶学性质及其对全麦面包品质的影响.

Published in:

Food Research & Development, 2022, v. 43, n. 11, p. 35, doi. 10.12161/j.issn.1005-6521.2022.11.005

By:

张亚萍;
赵风光;
刘纯;
杨曼丽;
林影;
韩双艳

Publication type:

Article

氨基甲酸乙酯水解酶异源表达及酶学性质分析.

Published in:

Food Research & Development, 2022, v. 43, n. 10, p. 195, doi. 10.12161/j.issn.1005-6521.2022.10.026

By:

高孝;
高洁;
刘晓宇;
王中选;
刘庆涛;
桑亚新

Publication type:

Article

黑木耳多糖的降解及其产物抗氧化性.

Published in:

Food Research & Development, 2022, v. 43, n. 10, p. 15, doi. 10.12161/j.issn.1005-6521.2022.10.003

By:

曹慧馨;
吴迪;
王旭升;
白洋;
吴琼;
代永刚

Publication type:

Article

Bacillus sphaericus DS11蛋白酶基因osp在 Bacillus subtilis的异源表达及酶学性质研究.

Published in:

Food Research & Development, 2020, v. 41, n. 12, p. 8, doi. 10.12161/j.issn.1005-6521.2020.12.002

By:

柴金龙;
胡晟源;
陈丽;
王淑军;
焦豫良;
杨杰;
刘姝;
房耀维

Publication type:

Article

Expression of Tailored α-N-Acetylglucosaminidase in Escherichia coli for Synthesizing Mannose-6-Phosphate on N-Linked Oligosaccharides of Lysosomal Enzymes.

Published in:

Bioengineering (Basel), 2025, v. 12, n. 4, p. 425, doi. 10.3390/bioengineering12040425

By:

Cao, Yunsong;
Wang, Wei

Publication type:

Article

A Cyber–Physical Production System for the Integrated Operation and Monitoring of a Continuous Manufacturing Train for the Production of Monoclonal Antibodies.

Published in:

Bioengineering (Basel), 2024, v. 11, n. 6, p. 610, doi. 10.3390/bioengineering11060610

By:

Thakur, Garima;
Nikita, Saxena;
Yezhuvath, Vinesh Balakrishnan;
Buddhiraju, Venkata Sudheendra;
Rathore, Anurag S.

Publication type:

Article

The Statistical Optimisation of Recombinant β-glucosidase Production through a Two-Stage, Multi-Model, Design of Experiments Approach.

Published in:

Bioengineering (Basel), 2019, v. 6, n. 3, p. 1, doi. 10.3390/bioengineering6030061

By:

Uhoraningoga, Albert;
Kinsella, Gemma K.;
Frias, Jesus M.;
Henehan, Gary T.;
Ryan, Barry J.

Publication type:

Article

Impact of Mobile Phase Parameters on Transport Properties of Metal Affinity Hydrogel Membranes.

Published in:

Separation Science & Technology, 2008, v. 43, n. 16, p. 4075, doi. 10.1080/01496390802414700

By:

Nave, F. M.;
Luo, Y. Z.;
Coleman, M. R.

Publication type:

Article

Development and Characterization of Affinity Membranes for Immunoglobulin Purification.

Published in:

Separation Science & Technology, 2007, v. 42, n. 13, p. 2987, doi. 10.1080/01496390701560140

By:

Boi, Cristiana;
Sarti, Giulio C.

Publication type:

Article