GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity.

Saleh, Mohammed S. M.; Bukhari, Dzatil Awanis Mohd; Siddiqui, Mohammad Jamshed Ahmad; Kasmuri, Abdul Razak; Murugesu, Suganya; Khatib, Alfi

Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ± 4.35%), whereas SFE_1 showed the lowest yield (0.42 ± 0.08%). All extracts were evaluated for in vitro α-glucosidase inhibitory activity, measured by their IC50 values in comparison to that of quercetin, the positive control (IC50 = 2.7 ± 0.7 μg/mL). The lowest α-glucosidase inhibitory activity was indicated by water extract of SE (IC50 = 724.3 ± 42.9 μg/mL) and the highest activity was demonstrated by 60% ethanol extract by UAE (IC50 = 16.2 ± 2.4 μg/mL). All extracts were analysed by GC-MS and identified metabolites like carbohydrates, fatty acids, organic acids, phenolic acids, sterols and alkane-based compounds etcetera that may possess the potential as α-glucosidase inhibitor and may attribute to the α-glucosidase inhibitory activity.

SUPERCRITICAL fluid extraction; METABOLITE analysis; ORGANIC acids; PHENOLIC acids; ORGANIC solvents

Natural Product Research, 2020, Vol 34, Issue 9, p1341

1478-6419

Academic Journal

10.1080/14786419.2018.1560295