Isolation and characterization of novel antibacterial compound from an untapped plant, Stereospermum fimbriatum.

Izyani Awang, Anis Fadhlina; Ahmed, Qamar Uddin; Shah, Syed Adnan Ali; Jaffri, Juliana Md.; Ghafoor, Kashif; Uddin, A. B. M. Helal; Ferdosh, Sahena; Islam Sarker, Md. Zaidul

Stereospermum fimbriatum or locally known as "Chicha" is traditionally used for itchy skin, earache, stomachache and postpartum treatments. This study was designed to evaluate the antimicrobial potential of S. fimbriatum's stem bark against 11 pathogens and isolate its bioactive compound. Successive soxhlet extraction was conducted using n-hexane, dichloromethane (DCM) and methanol. Disc diffusion, minimum inhibitory and bactericidal concentration (MIC & MBC) assays were done to examine the antimicrobial activity. Bioassay-guided isolation was conducted on S. fimbriatum's extract. The DCM extract of stem bark (DS) was the most potent extract followed by n-hexane extract of the stem bark (NS). A novel compound was isolated and coded as C1 which demonstrated potent antibacterial effects with the MIC values as low as 3.13 µg/mL to 6.25 µg/mL, against S. epidermidis, MRSA and S. aureus. Thus, S. fimbriatum could be a potential source of antimicrobial agents for the treatment of skin infections, specifically, MRSA.

DISC diffusion tests (Microbiology); DICHLOROMETHANE; SKIN infections; ANTI-infective agents; EARACHE; ABDOMINAL pain; BIOACTIVE compounds

Natural Product Research, 2020, Vol 34, Issue 5, p629

1478-6419

Academic Journal

10.1080/14786419.2018.1494170