Results
Title

Tricin isolated from Allium atroviolaceum potentiated the effect of docetaxel on PC3 cell proliferation: role of miR-21.

Authors

Ghasemi, Sorayya; Lorigooini, Zahra; Wibowo, JokoPriyanto; Amini-khoei, Hossein

Abstract

For more effectiveness and overcoming the drug resistance Chemotherapy agents, the combination treatment is raised. Flavonoids with different anti-cancer effects are an appropriate choice as lead compounds. Over expressed MiR-21 in prostate cancer is associated with metastasis and drug resistance to chemotherapy with Docetaxel. In this study, the anticancer effect of 4′, 5, 7-Trihydroxy-3′, 5′-dimethoxyflavone (Tricin) was investigated with Docetaxel on PC3 cell line. Tricin was initially isolated from the Allium atroviolaceum by column chromatography and recrystallization method. The chemical structure of isolate was elucidated by spectroscopic techniques. IC50 of Tricin and Docetaxel were assessed 117.5 ± 4.4 μM and 0.1 ± 0.02 nM by MTT assay, respectively. Analysis of results indicates the synergistic effect of combination therapy on decreased proliferation. MiR-21 in treated cells with Tricin significantly decreased compared to control cells. So, Tricin can be effective in the reduction of metastasis and drug resistance of Docetaxel.

Subjects

DOCETAXEL; PROSTATE cancer treatment; DRUG resistance; CANCER chemotherapy; CELL proliferation

Publication

Natural Product Research, 2019, Vol 33, Issue 12, p1828

ISSN

1478-6419

Publication type

Academic Journal

DOI

10.1080/14786419.2018.1437439