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- Title
Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography.
- Authors
Lu, Lu; Liu, Yanping; Ding, Yue; Hou, Jianwei; Zhang, Yong; Xue, Haiping; Zhang, Tong
- Abstract
Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed.β-D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7 g of TAIII with a high purity of > 97% was obtained from 1 kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale.
- Publication
Natural Product Research, 2016, Vol 30, Issue 20, p2364
- ISSN
1478-6419
- Publication type
Academic Journal
- DOI
10.1080/14786419.2016.1169416