A new oleanane-type triterpenoidal saponin, 3-O-β-d-glucopyranosyl-hederagenin 23-O-α-d-ribofuranoside (1), was isolated from the roots of Pulsatilla chinensis, and its structure was established on the basis of the spectral data. Compounds 2, 7 and 8 showed moderate cytotoxic activity by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide assay.