The volatile constituents of Annona squamosa L. bark were identified from the essential oil obtained by steam distillation and studied by GC/MS. Six major components were identified as 1H-Cycloprop(e)azulene (3.46%), germacrene D (11.44%), bisabolene (4.48%), caryophyllene oxide (29.38%), bisabolene epoxide (3.64%) and kaur-16-ene (19.13%). The oil was also screened for its antimicrobial activity, which exhibited a significant antimicrobial activity against Bacillus subtilis and Staphylococcus aureus .