Twenty three samples of 18 Portuguese olive leaf cultivars were analysed by a reversed-phase HPLC/DAD procedure and eight flavonoidic compounds were identified and quantified (luteolin 7,4′- O -diglucoside, luteolin 7- O -glucoside, rutin, apigenin 7- O -rutinoside, luteolin 4′- O -glucoside, luteolin, apigenin and diosmetin). Luteolin 7,4′- O -diglucoside and luteolin 4′- O -glucoside were identified by HPLC/DAD/MS/MS - ESI. The studied olive leaf samples showed a common phenolic pattern, in which luteolin 4′- O -glucoside was almost always the major compound.